Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD3 epsilon with ab237707 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human tonsil is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab237707 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling CD3 epsilon with ab237707 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the infiltrating T lymphocytes in the human gastric carcinoma is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab237707 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD3 epsilon with ab237707 at 1/55 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

PBS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

CD3 epsilon was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab237707 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237707 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000  dilution.

Lane 1: Jurkat whole cell lysate 10 μg (Input).
Lane 2: ab237707 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237707 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.

Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20 permeabilized human PBMCs (peripheral blood mononuclear cells) labeling CD3 epsilon with ab237707 at 1/500 (Right compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat Anti-Rabbit IgG Fc (Alexa Fluor^® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with anti-CD3 epsilon conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining rabbit IgG (Left) or ab237707 (Right).

CD3 epsilon was immunoprecipitated from 0.35 mg of human thymus lysate with ab237707 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237707 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000  dilution.

Lane 1: Human thymus lysate 10 μg (Input). 
Lane 2: ab237707 IP in human thymus lysate. 
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237707 in human thymus lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.

Formalin-fixed, paraffin-embedded human NSCLC tissue stained for CD3 epsilon using ab237707 at 0.5 µg/mL in immunohistochemcial analysis.

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3 epsilon using ab237707 at 0.5 µg/mL in immunohistochemcial analysis.