Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)
![Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)](https://www.abcam.com/ps/products/236/ab236155/Images/ab236155-325059-anti-cd27-antibody-epr20585-immunohistochemistry.jpg "Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20585] to CD27 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-CD27 antibody [EPR20585] - BSA and Azide free
See all CD27 primary antibodies -
Description
Rabbit monoclonal [EPR20585] to CD27 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse spleen tissue.
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General notes
Ab236155 is the carrier-free version of ab214043. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236155 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20585 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD27 with ab214043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse thymus (PMID: 11728341). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214043).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)](https://www.abcam.com/ps/products/236/ab236155/Images/ab236155-311883-anti-cd27-antibody-epr20585-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD27 with ab214043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 11782274, PMID: 11728341). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214043).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)](https://www.abcam.com/ps/products/236/ab236155/Images/ab236155-3-benefits-of-recombinant-antibodies.png)
Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)
