Recombinant human CD19 protein (Fc Chimera Active) (FITC) (ab246020)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
- Antibody deficiency due to defect in CD19
- Antibody deficiency due to defect in CD19, included
- AW495831
Images
-
Functional Studies - Recombinant human CD19 protein (Fc Chimera Active) (FITC) (ab246020)
HEK-293 (Human epithelial cell line from embryonic kidney) cells were transfected with CAR (anti-CD19-scFv and RFP tag). 2 x 105 of the cells were stained with ab246020 (10 µg/ml) (Panel B) or an FITC-labeled protein control (Panel C). Non-transfected HEK-293 cells were also stained with ab246020 (Panel A). FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of ab246020.
-
SDS-PAGE - Recombinant human CD19 protein (Fc Chimera Active) (FITC) (ab246020)SDS-PAGE - Recombinant human CD19 protein (Fc Chimera Active) (FITC) (ab246020).
The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. The protein migrates as 70 kDa and 150 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
