Mouse ST6Gal1 ELISA Kit (ab275104)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.53 pg/ml
- Range: 46.8 pg/ml - 3000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse ST6Gal1 ELISA Kit
See all CD75 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.9% Inter-assay Sample n Mean SD CV% Serum 3 9.1% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
27.53 pg/ml -
Range
46.8 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99 92% - 106% Hep Plasma 105 97% - 117% EDTA Plasma 101 83% - 114% Cit plasma 92 89% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse ST6Gal1 ELISA kit (ab275104) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse ST6Gal1 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ST6Gal1 with 27.53 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ST6GAL1 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Literature has sited ST6GAL1 is expressed in mouse high endothelial cells of mesenteric lymph nodes and Peyer's patches. This kit recognizes the soluble form of mouse ST6Gal1 protein. Mouse ST6GAL1 shares 95%, 81%, 81%, 76% sequence homology with rat, human, primate, and cow, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse ST6Gal1 Capture Antibody 1 x 600µl 10X Mouse ST6Gal1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse ST6Gal1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 29 family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. - Information by UniProt
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Alternative names
- 6 sialyltransferase
- 6-sialyltransferase 1
- 6-ST 1
see all -
Database links
- Entrez Gene: 20440 Mouse
- SwissProt: Q64685 Mouse
- Unigene: 149029 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse ST6Gal1 standard curve in Sample Diluent NThe ST6Gal1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ST6Gal1 in mouse serum and plasma samples.The concentrations of ST6Gal1 were measured in duplicates, interpolated from the ST6Gal1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST6Gal1 concentration was determined to be 5.53 ng/mL in serum, 7.66 ng/mL in plasma (citrate), 5.00 ng/mL in, plasma (EDTA), and 4.15 ng/mL in plasma (heparin).
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Sandwich ELISA - Mouse ST6Gal1 ELISA Kit (ab275104)To learn more about the advantages of recombinant antibodies see here.
