Anti-SF2 antibody (ab238523)
Key features and details
- Rabbit polyclonal to SF2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SF2 antibody
See all SF2 primary antibodies -
Description
Rabbit polyclonal to SF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
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Immunogen
Fusion protein corresponding to Human SF2 aa 1-250. NP_008855.1
Database link: Q07955 -
Positive control
- WB: HeLa, MCF7 and DU 145 cell extracts. Mouse spleen and thymus extracts. IHC-P: Rat lung tissue. Mouse esophagus tissue. Human lung cancer tissue. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody (ab238523)
U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for SF2 using ab238523 (Top panel, red) at a 1/50 dilution in ICC/IF. Counterstained with DAPI (Bottom panel, merge).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody (ab238523)Paraffin-embedded human lung cancer tissue stained for SF2 with ab238523 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody (ab238523)Paraffin-embedded mouse esophagus tissue stained for SF2 with ab238523 at a 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-SF2 antibody (ab238523)All lanes : Anti-SF2 antibody (ab238523) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lane 3 : DU 145 (Human prostate carcinoma cell line) cell extract
Lane 4 : Mouse thymus extract
Lane 5 : Mouse spleen extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDaBlocking buffer: 3% non-fat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody (ab238523)Paraffin-embedded rat lung tissue stained for SF2 with ab238523 at a 1/100 dilution in immunohistochemical analysis.
