Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9864] to KHSRP - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KHSRP antibody [EPR9864] - BSA and Azide free
    See all KHSRP primary antibodies

  • Description

    Rabbit monoclonal [EPR9864] to KHSRP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248962 is the carrier-free version of ab150393. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248962 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9864

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    Western blot - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    All lanes : Anti-KHSRP antibody [EPR9864] (ab150393) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A673 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 73 kDa



    This data was developed using ab150393, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    This data was developed using ab150393, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling KHSRP with ab150393 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    Flow Cytometry - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    This data was developed using ab150393, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KHSRP (red) with purified ab150393 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    This data was developed using ab150393, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling KHSRP with ab150393 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    Immunocytochemistry - Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    This data was developed using ab150393, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labelling KHSRP with ab150393 at 1/100 dilution.
  • Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)
    Anti-KHSRP antibody [EPR9864] - BSA and Azide free (ab248962)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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