Anti-Dnmt3a antibody - N-terminal (ab227725)
Key features and details
- Rabbit polyclonal to Dnmt3a - N-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Dnmt3a antibody - N-terminal
See all Dnmt3a primary antibodies -
Description
Rabbit polyclonal to Dnmt3a - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human Dnmt3a (N terminal). The exact sequence is proprietary.
Database link: Q9Y6K1 -
Positive control
- WB: HEK-293T, Neuro-2a, PC-12 and Rat2 whole cell extracts; HEK-293T nuclear extract. IP: HEK-293T whole cell and nuclear extracts.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725)All lanes : Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/3000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Dnmt3a shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.5% SDS-PAGE gel.
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Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725)All lanes : Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract
Lysates/proteins at 30 µg per lane.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab227725)Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract with ab227725. Western blot was performed from the immunoprecipitate using ab227725 at 1/2000 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T nuclear extract 40 µg (Input).
Lane 2: 4 µg preimmune rabbit IgG instead of ab227725 in HEK-293T nuclear extract.
Lane 3: ab227725 IP in HEK-293T nuclear extract.
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Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725)Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with ab227725. Western blot was performed from the immunoprecipitate using ab227725. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell exract (Input).
Lane 2: Control IgG instead of ab227725 in HEK-293T whole cell extract.
Lane 3: ab227725 IP in HEK-293T whole cell extract.
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Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725)Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG7.5% SDS-PAGE gel.
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Western blot - Anti-Dnmt3a antibody - N-terminal (ab227725)All lanes : Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG7.5% SDS-PAGE gel.
