Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGMT antibody [MT3.1] (ab39253)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [MT3.1] to MGMT
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MGMT antibody [MT3.1]
    See all MGMT primary antibodies

  • Description

    Mouse monoclonal [MT3.1] to MGMT

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human MGMT.

  • Positive control

    • IHC-P: Human prostate carcinoma and lung carcinoma tissue. WB: MCF7, HeLa, Jurkat and HCT116 whole cell lysate. Wild type HAP1 whole cell lysate.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 02nd November 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-MGMT antibody [MT3.1] (ab39253)
    Western blot - Anti-MGMT antibody [MT3.1] (ab39253)
    All lanes : Anti-MGMT antibody [MT3.1] (ab39253) at 1/500 dilution

    Lane 1 : Wild type HAP1 whole cell lysate
    Lane 2 : MGMT knockout HAP1 whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell)
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] (ab39253)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] (ab39253)

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue labeling MGMT with ab39253 at 1/200 followed by a ready to use secondary. Positive staining on human prostate carcinoma. The section was incubated with ab39253 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use secondary. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] (ab39253)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] (ab39253)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling MGMT with ab39253 at 1/200 followed by a ready to use secondary. Positive staining on human lung carcinoma. The section was incubated with ab39253 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use secondary. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-MGMT antibody [MT3.1] (ab39253)
    Western blot - Anti-MGMT antibody [MT3.1] (ab39253)
    All lanes : Anti-MGMT antibody [MT3.1] (ab39253) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 92 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] (ab39253)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] (ab39253) This image is courtesy of an anonymous abreview.

    ab39253 staining MGMT in human stomach tissue sections by Immunohistochemistry. Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH6.0. Samples were then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody, at a 1/100 dilution, for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.

    This image was generated from the hybridoma version.

    See Abreview

  • Western blot - Anti-MGMT antibody [MT3.1] (ab39253)
    Western blot - Anti-MGMT antibody [MT3.1] (ab39253) This image is courtesy of an anonymous Abreview
    All lanes : Anti-MGMT antibody [MT3.1] (ab39253) at 1/500 dilution (for 16 hours at 4°C)

    All lanes : Human OE33 cells - whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking Step: 5% BSA for 1 hour at 23°C

    This image was generated from the hybridoma version.

    See Abreview

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