DNMT1 Assay Kit (ab113469)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell Lysate, Tissue Extracts
Overview
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Product name
DNMT1 Assay Kit -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity (quantitative) -
Assay time
3h 00m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.
DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 48 tests 10X Wash Buffer 1 x 28ml 1 x 14ml 8-Well Assay Strips (with Frame) 12 units 6 units Capture Antibody, 500 µg/mL 1 x 16µl 1 x 8µl Assay Buffer 1 x 10ml 1 x 5ml Blocking Buffer 1 x 20ml 1 x 10ml Detection Antibody, 200 µg/mL 1 x 20µl 1 x 10µl Developing Solution 1 x 12ml 1 x 6ml DNMT1 Standard, 20 µg/mL 1 x 40µl 1 x 20µl Stop Solution 1 x 11ml 1 x 6ml -
Research areas
Images
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Functional Studies - DNMT1 Assay Kit (ab113469)