Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGMT antibody [EPR4397] (ab108630)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4397] to MGMT
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MGMT antibody [EPR4397]
    See all MGMT primary antibodies

  • Description

    Rabbit monoclonal [EPR4397] to MGMT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, Jurkat, HeLa, and HT29 cell lysates IHC-P: Human clear cell carcinoma, lung cancer, and spleen tissues

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-MGMT antibody [EPR4397] (ab108630)
    Western blot - Anti-MGMT antibody [EPR4397] (ab108630)
    Anti-MGMT antibody [EPR4397] (ab108630) at 1/5000 dilution (Purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Western blot - Anti-MGMT antibody [EPR4397] (ab108630)
    Western blot - Anti-MGMT antibody [EPR4397] (ab108630)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MGMT knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab108630 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108630 was shown to specifically react with MGMT when MGMT knockout samples were used. Wild-type and MGMT knockout samples were subjected to SDS-PAGE. ab108630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [EPR4397] (ab108630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [EPR4397] (ab108630)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling MGMT with purified ab108630 at 1:800 dilution (0.94 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-MGMT antibody [EPR4397] (ab108630)
    Western blot - Anti-MGMT antibody [EPR4397] (ab108630)
    All lanes : Anti-MGMT antibody [EPR4397] (ab108630) at 1/1000 dilution (Unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : Jurkat ell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HT29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [EPR4397] (ab108630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [EPR4397] (ab108630)

    Unpurified ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human clear cell carcinoma tissue by Immunohistochemistry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [EPR4397] (ab108630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [EPR4397] (ab108630)

    Unpurified ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human spleen tissue by Immunohistochemistry.

  • Anti-MGMT antibody [EPR4397] (ab108630)
    Anti-MGMT antibody [EPR4397] (ab108630)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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