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Anti-MRPL48 antibody [EPR16328] (ab194826)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16328] to MRPL48
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MRPL48 antibody [EPR16328]
    See all MRPL48 primary antibodies

  • Description

    Rabbit monoclonal [EPR16328] to MRPL48

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, HeLa, PC12, NIH 3T3, Jurkat, Human fetal heart lysate; mouse brain, mouse heart, rat brain and rat heart lysates; Human cervix carcinoma tissue; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MRPL48 antibody [EPR16328] (ab194826)
    Western blot - Anti-MRPL48 antibody [EPR16328] (ab194826)
    All lanes : Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL48 antibody [EPR16328] (ab194826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL48 antibody [EPR16328] (ab194826)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling MRPL48 with ab194826 at 1/150 dilution. A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194826. Cytoplasm staining on human cervix carcinoma tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MRPL48 antibody [EPR16328] (ab194826)
    Immunocytochemistry/ Immunofluorescence - Anti-MRPL48 antibody [EPR16328] (ab194826)

    Immunofluorescent analysis of A431 cells labeling MRPL48 with ab194826 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HepG2 cell line was observed.

  • Western blot - Anti-MRPL48 antibody [EPR16328] (ab194826)
    Western blot - Anti-MRPL48 antibody [EPR16328] (ab194826)
    Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/2000 dilution + Human fetal heart lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Flow Cytometry - Anti-MRPL48 antibody [EPR16328] (ab194826)
    Flow Cytometry - Anti-MRPL48 antibody [EPR16328] (ab194826)

    Flow cytometry analysis of HeLa cells labelling MRPL48 (red) with purified ab194826 at dilution of 1/60. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Western blot - Anti-MRPL48 antibody [EPR16328] (ab194826)
    Western blot - Anti-MRPL48 antibody [EPR16328] (ab194826)
    All lanes : Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunoprecipitation - Anti-MRPL48 antibody [EPR16328] (ab194826)
    Immunoprecipitation - Anti-MRPL48 antibody [EPR16328] (ab194826)

    Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab194826 at 1/30 dilution (lane 1) or PBS control (lane 2)

    For Western blot: ab194826 was used at 1/1000 dilution (0.6µg/ml). Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-MRPL48 antibody [EPR16328] (ab194826)
    Anti-MRPL48 antibody [EPR16328] (ab194826)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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