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Epigenetics and Nuclear Signaling DNA / RNA Translation Mito. Translation

Anti-GFM1 antibody [EPR12263-33] (ab173529)

Price and availability

324 988 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-GFM1 antibody [EPR12263-33] (ab173529)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12263-33] to GFM1
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-GFM1 antibody [EPR12263-33]
    See all GFM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12263-33] to GFM1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human GFM1. The exact sequence is proprietary.
    Database link: Q96RP9

  • Positive control

    • HeLa, NCI-H460, U87-MG and 293T cell lysates. HeLa cells. Immunoprecipitation pellet from HeLa whole cell lysate (ab150035).
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12263-33
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Mito. Translation
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism

Images

  • Western blot - Anti-GFM1 antibody [EPR12263-33] (ab173529)
    Western blot - Anti-GFM1 antibody [EPR12263-33] (ab173529)
    All lanes : Anti-GFM1 antibody [EPR12263-33] (ab173529) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : NCI-H460 cell lysates
    Lane 3 : U87-MG cell lysates
    Lane 4 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 83 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GFM1 antibody [EPR12263-33] (ab173529)
    Immunocytochemistry/ Immunofluorescence - Anti-GFM1 antibody [EPR12263-33] (ab173529)

    Immunofluorescence analysis of HeLa cells labeling GFM1 with ab173529 at a 1/50 dilution.

  • Immunoprecipitation - Anti-GFM1 antibody [EPR12263-33] (ab173529)
    Immunoprecipitation - Anti-GFM1 antibody [EPR12263-33] (ab173529)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate: ab173529 at a 1/10 dilution was used for immunoprecipitate GFM1.

  • Anti-GFM1 antibody [EPR12263-33] (ab173529)
    Anti-GFM1 antibody [EPR12263-33] (ab173529)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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