Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17046] to U2AF65 - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-U2AF65 antibody [EPR17046] - C-terminal
    See all U2AF65 primary antibodies

  • Description

    Rabbit monoclonal [EPR17046] to U2AF65 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293, Jurkat, HeLa, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. Mouse brain lysates. IHC: Human endometrial adenocarcinoma tissue, Mouse liver tissue and Rat cerebral cortex tissue. ICC/IF: SW480 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17046

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    All lanes : Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/20000 dilution

    Lane 1 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Immunocytochemistry/ Immunofluorescence - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling U2AF65 with Purified ab197031 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human endometrial adenocarcinoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    All lanes : Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Western blot - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/2000 dilution + Mouse brain lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Mouse liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat cerebral cortex tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)
    Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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