Anti-NOP10 antibody [EPR8856] (ab133726)
![Anti-NOP10 antibody [EPR8856] (ab133726)](https://www.abcam.com/ps/products/133/ab133726/Images/ab133726-147777-anti-nop10-antibody-epr8856-immunohistochemistry.jpg "Anti-NOP10 antibody [EPR8856] (ab133726)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8856] to NOP10
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-NOP10 antibody [EPR8856]
See all NOP10 primary antibodies -
Description
Rabbit monoclonal [EPR8856] to NOP10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human NOP10 aa 1-100. The exact sequence is proprietary.
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Positive control
- Human breast and papillary adenocarcinoma tissues; Jurkat, MOLT4, Raji and HeLa cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8856 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8856] (ab133726)
Immunohistochemical analysis of paraffin-embedded Human breast tissue staining NOP10 using ab133726 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-NOP10 antibody [EPR8856] (ab133726)](https://www.abcam.com/ps/products/133/ab133726/Images/ab133726-147778-anti-nop10-antibody-epr8856-western-blot.jpg)
Western blot - Anti-NOP10 antibody [EPR8856] (ab133726)All lanes : Anti-NOP10 antibody [EPR8856] (ab133726) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 7 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8856] (ab133726)](https://www.abcam.com/ps/products/133/ab133726/Images/ab133726-147776-anti-nop10-antibody-epr8856-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8856] (ab133726)Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma tissue staining NOP10 using ab133726 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-NOP10 antibody [EPR8856] (ab133726)](https://www.abcam.com/ps/products/133/ab133726/Images/ab133726-147775-anti-nop10-antibody-epr8856.jpg)
OI-RD Scanning - Anti-NOP10 antibody [EPR8856] (ab133726) -
![Anti-NOP10 antibody [EPR8856] (ab133726)](https://www.abcam.com/ps/products/133/ab133726/Images/ab133726-5-benefits-of-recombinant-antibodies.png)
Anti-NOP10 antibody [EPR8856] (ab133726)
