Anti-NOP10 antibody [EPR8857] (ab134902)
![Anti-NOP10 antibody [EPR8857] (ab134902)](https://www.abcam.com/ps/products/134/ab134902/Images/ab134902-149021-anti-nop10-antibody-epr8857-immunohistochemistry.jpg "Anti-NOP10 antibody [EPR8857] (ab134902)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8857] to NOP10
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-NOP10 antibody [EPR8857]
See all NOP10 primary antibodies -
Description
Rabbit monoclonal [EPR8857] to NOP10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues near the C terminus of Human NOP10 (UniProt: Q9NPE3).
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Positive control
- 293T, HeLa, Jurkat, HuT-78, and Caco 2 cell lysates. Human breast and kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.50 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8857 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8857] (ab134902)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling NOP10 with ab134902 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-NOP10 antibody [EPR8857] (ab134902)](https://www.abcam.com/ps/products/134/ab134902/Images/ab134902-149022-anti-nop10-antibody-epr8857-western-blot.jpg)
Western blot - Anti-NOP10 antibody [EPR8857] (ab134902)All lanes : Anti-NOP10 antibody [EPR8857] (ab134902) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HuT-78 cell lysate
Lane 5 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution
Predicted band size: 7 kDa
Observed band size: 8 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8857] (ab134902)](https://www.abcam.com/ps/products/134/ab134902/Images/ab134902-149020-anti-nop10-antibody-epr8857-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP10 antibody [EPR8857] (ab134902)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling NOP10 with ab134902 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-NOP10 antibody [EPR8857] (ab134902)](https://www.abcam.com/ps/products/134/ab134902/Images/ab134902-149019-anti-nop10-antibody-epr8857.jpg)
OI-RD Scanning - Anti-NOP10 antibody [EPR8857] (ab134902) -
![Anti-NOP10 antibody [EPR8857] (ab134902)](https://www.abcam.com/ps/products/134/ab134902/Images/ab134902-5-benefits-of-recombinant-antibodies.png)
Anti-NOP10 antibody [EPR8857] (ab134902)
