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Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15112(B)] to ALAS2/ASB - C-terminal
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal
    See all ALAS2/ASB primary antibodies

  • Description

    Rabbit monoclonal [EPR15112(B)] to ALAS2/ASB - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3, PC-12, Rat brain, Jurkat, 293 and Human fetal liver. IP: K-562 cells.

  • General notes

     This product was previously labelled as ALAS2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    All lanes : Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964) at 1/1000 dilution (Purified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    ab184964 (Purified) at 1:30 dilution (2 µg) immunoprecipitating ALAS2/ASB in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
    Lane 2 (+): ab184964 & K-562 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab184964 in K-562 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    All lanes : Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964) at 1/1000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Jurkat lysate
    Lane 3 : 293 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 65 kDa

  • Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    Western blot - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964) at 1/1000 dilution + Human fetal liver at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 65 kDa

  • Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    Immunoprecipitation - Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)

    Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab184964 at 1/50 dilution. 

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.

  • Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)
    Anti-ALAS2/ASB antibody [EPR15112(B)] - C-terminal (ab184964)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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