Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Human HDAC8 Antibody Pair - BSA and Azide free (ab253554)

Price and availability

670 ₸

Availability

Order now and get it on Wednesday March 24, 2021

Human HDAC8 Antibody Pair - BSA and Azide free (ab253554)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

You may also be interested in

Product image
Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482)
Product image
Human HDAC8 knockout HeLa cell lysate (ab257249)
Product image
Anti-Ctip2 antibody [EPR12275(2)] - BSA and Azide free (ab243191)
Product image
Anti-Ctip1/BCL-11A antibody (ab245558)

Overview

  • Product name

    Human HDAC8 Antibody Pair - BSA and Azide free
    See all HDAC8 kits
  • Assay type

    ELISA set
  • Range

    64 pg/ml - 4000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview


    • Human HDAC8 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human HDAC8 in sandwich ELISAs and many other pair-based applications.

    • The pair can be used in variety of assays and platforms including but not limited to:

    •      - Sandwich ELISA

    •      - FRET/TR-FRET/HTR

    •      - Meso Scale Discovery® (MSD®)

    •      - Luminex® and bead-based assays

    •      - AlphaLISA®/AlphaScreen®

    •      - DELFIA® immunoassays

    •      - Simoa® and Single Molecule Counting (SMC™) immunoassays

    •      - Multiplex

    • Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

    •      - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.

    •      - Concentration of ~1 mg/ml as measured by the protein A280 method.

    • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
    • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

    • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

    • Please note:

    • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

    • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

    • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

    •  Antibody properties:

    •  Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

    •  Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

    •  Concentration: ~1 mg/ml

    •  Storage buffer: 100% PBS

    •  Form: Liquid

    •  Isotype: IgG

    •  Recombinant monoclonal antibodies offer several advantages including:

    •      - High batch-to-batch consistency and reproducibility

    •      - Improved sensitivity and specificity

    •      - Long-term security of supply

    •      - Animal-free production

    • For more information see here.

    • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

    • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.

    • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

    • Simoa is a registered trademark of Quanterix, Inc.

    • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components Identifier 10 x 96 tests
    Human HDAC8 Capture Antibody (unconjugated) —
    Human HDAC8 Detector Antibody (unconjugated) —
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificity

    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localization

    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Target information above from: UniProt accession Q9BY41 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CDA07
    • CDLS5
    • HD 8
    • HD8
    • HDAC 8
    • Hdac8
    • HDAC8_HUMAN
    • HDACL 1
    • HDACL1
    • Histone deacetylase 8
    • Histone deacetylase like 1
    • MRXS6
    • RPD 3
    • RPD3
    • WTS
    see all
  • Database links

    • Entrez Gene: 55869 Human
    • Omim: 300269 Human
    • SwissProt: Q9BY41 Human
    • Unigene: 310536 Human

    Images

    • Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253554)
      Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253554)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Clear all

    Recently viewed products

    •  
    • Product image

      Human CXCL5 ELISA Kit (ab212163)

    •  
    • Product image

      Anti-Alpha Skeletal Muscle Actin antibody (ab235928)

    •  
    • Product image

      PE Anti-HDAC2 antibody [Y461] (ab210827)

    Get resources and offers direct to your inbox Sign up
    © 2021 Astana Biomed Group LLP. All rights reserved.