Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5223] to HDAC9 - BSA and Azide free
  • Suitable for: ICC, IP, WB, IHC-P, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HDAC9 antibody [EPR5223] - BSA and Azide free
    See all HDAC9 primary antibodies

  • Description

    Rabbit monoclonal [EPR5223] to HDAC9 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the IHC-P results. We do not guarantee WB for mouse and rat.

  • Tested applications

    Suitable for: ICC, IP, WB, IHC-P, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HAP1 and Raji whole cell lysate.

  • General notes

    ab239979 is the carrier-free version of ab109446.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

    This data was developed using ab109446, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: HDAC9 (KO) knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HepG2 whole cell lysate (20 µg)

    Lane 4: Raji whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109446 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109446 was shown to specifically recognize HDAC9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HDAC9 knockout samples were usedexamined. Wild-type and HDAC9 knockout samples were subjected to SDS-PAGE. ab109446 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    This data was developed using ab109446, the same antibody clone in a different buffer formulation.ab109446 at 1/250 dilution staining HDAC9 in Human colon by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    Immunocytochemistry - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    This data was developed using ab109446, the same antibody clone in a different buffer formulation.ab109446 at 1/100 dilution staining HDAC9 in HeLa cells by Immunofluorescence.
  • Flow Cytometry - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    Flow Cytometry - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    This data was developed using ab109446, the same antibody clone in a different buffer formulation.Flow cytometry of K-562 Human chronic myelogenous leukemia lymphoblast cells staining HDAC9 with ab109446 (red) at 1:250. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used at 1:2000 as a secondary. Rabbit monoclonal IgG (ab172730) (Black) was used as an isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) used as an unlabelled control. Fixed with 4% paraformaldehyde. Permeabilised with 90% methanol.
  • Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    All lanes : Anti-HDAC9 antibody [EPR5223] (ab109446) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 111 kDa
    Observed band size: 160 kDa why is the actual band size different from the predicted?



    This data was developed using ab109446, the same antibody clone in a different buffer formulation.

     

  • Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
    Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

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