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Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16518] to Prostacyclin receptor
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Prostacyclin receptor antibody [EPR16518]
    See all Prostacyclin receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR16518] to Prostacyclin receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 whole cell lysate (ab7900); Human fetal kidney lysate; Human endometrium tissue; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
    Western blot - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
    All lanes : Anti-Prostacyclin receptor antibody [EPR16518] (ab196653) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Prostacyclin receptor with ab196653 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cell membrane staining on Human endometrium is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
    Flow Cytometry - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma) cells labeling Prostacyclin receptor with ab196653 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
    Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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