Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16518] to Prostacyclin receptor
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-Prostacyclin receptor antibody [EPR16518]
See all Prostacyclin receptor primary antibodies
Description

Rabbit monoclonal [EPR16518] to Prostacyclin receptor
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2 whole cell lysate (ab7900); Human fetal kidney lysate; Human endometrium tissue; HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16518
Isotype

IgG
Research areas

Western blot - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

All lanes : Anti-Prostacyclin receptor antibody [EPR16518] (ab196653) at 1/1000 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Prostacyclin receptor with ab196653 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cell membrane staining on Human endometrium is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma) cells labeling Prostacyclin receptor with ab196653 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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