Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
![Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)](https://www.abcam.com/ps/products/172/ab172614/Images/ab172614-195655-anti-u2af35-antibody-epr12649b-western-blot.jpg "Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12649(B)] to U2AF35/U2AF1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-U2AF35/U2AF1 antibody [EPR12649(B)]
See all U2AF35/U2AF1 primary antibodies -
Description
Rabbit monoclonal [EPR12649(B)] to U2AF35/U2AF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
within Human U2AF35/U2AF1 aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q01081 -
Positive control
- HeLa, 293T, Ramos and NIH 3T3 cell lysates; HeLa cells; Human endometrial carcinoma and Human pancreas tissues.
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General notes
This product was previously labelled as U2AF35
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12649(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)All lanes : Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)](https://www.abcam.com/ps/products/172/ab172614/Images/ab172614-195657-anti-u2af35-antibody-epr12649b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)](https://www.abcam.com/ps/products/172/ab172614/Images/ab172614-195656-anti-u2af35-antibody-epr12649b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)](https://www.abcam.com/ps/products/172/ab172614/Images/ab172614-195659-anti-u2af35-antibody-epr12649b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)Immunofluorescent analysis of HeLa cells labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.
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![Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)](https://www.abcam.com/ps/products/172/ab172614/Images/ab172614-195658-anti-u2af35-antibody-epr12649b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab172614 at 1/10 dilution.
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![Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)](https://www.abcam.com/ps/products/172/ab172614/Images/ab172614-6-benefits-of-recombinant-antibodies.png)
Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
