Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)
![Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)](https://www.abcam.com/ps/products/197/ab197591/Images/ab197591-240109-anti-u2af35-antibody-epr126482-western-blot.jpg "Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12648(2)] to U2AF35/U2AF1
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-U2AF35/U2AF1 antibody [EPR12648(2)]
See all U2AF35/U2AF1 primary antibodies -
Description
Rabbit monoclonal [EPR12648(2)] to U2AF35/U2AF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P RatHumanWB RatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos, HeLa and 293 whole cell lysates and rat spleen tissue lysate. IHC-P: Human transitional cell carcinoma of bladder and rat liver tissues. ICC/IF: Ramos cells. Flow Cyt: Ramos cells.
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General notes
This product was previously labelled as U2AF35
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12648(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/10000 dilution + Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID:1388271.
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![Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)](https://www.abcam.com/ps/products/197/ab197591/Images/ab197591-240108-anti-u2af35-antibody-epr126482-western-blot.jpg)
Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)All lanes : Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/20000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID:1388271.
-
![Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)](https://www.abcam.com/ps/products/197/ab197591/Images/ab197591-240107-anti-u2af35-antibody-epr126482-western-blot.jpg)
Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/1000 dilution + Rat spleen lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID:1388271
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)](https://www.abcam.com/ps/products/197/ab197591/Images/ab197591-240106-anti-u2af35-antibody-epr126482-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling U2AF35/U2AF1 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)](https://www.abcam.com/ps/products/197/ab197591/Images/ab197591-240105-anti-u2af35-antibody-epr126482-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U2AF35/U2AF1 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP)(ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on rat liver tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)](https://www.abcam.com/ps/products/197/ab197591/Images/ab197591-261612-anti-u2af35-antibody-epr126482-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)Immunocytochemistry/Immunofluorescence analysis of RAMOS cells labelling U2AF35/U2AF1 with ab197591 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
![Flow Cytometry - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)](https://www.abcam.com/ps/products/197/ab197591/Images/ab197591-240102-anti-u2af35-antibody-epr126482-flow-cytometry.jpg)
Flow Cytometry - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos (Human Burkitt's lymphoma) cells labeling U2AF35/U2AF1 with ab197591 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)](https://www.abcam.com/ps/products/197/ab197591/Images/ab197591-10-benefits-of-recombinant-antibodies.png)
Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)
