Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

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Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12648(2)] to U2AF35/U2AF1
Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
Reacts with: Rat, Human

Product name

Anti-U2AF35/U2AF1 antibody [EPR12648(2)]
See all U2AF35/U2AF1 primary antibodies
Description

Rabbit monoclonal [EPR12648(2)] to U2AF35/U2AF1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Rat Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Ramos, HeLa and 293 whole cell lysates and rat spleen tissue lysate. IHC-P: Human transitional cell carcinoma of bladder and rat liver tissues. ICC/IF: Ramos cells. Flow Cyt: Ramos cells.
General notes
This product was previously labelled as U2AF35

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12648(2)
Isotype

IgG
Research areas

Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/10000 dilution + Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID:1388271.
Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

All lanes : Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/20000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293 (Human embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID:1388271.
Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/1000 dilution + Rat spleen lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID:1388271
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling U2AF35/U2AF1 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U2AF35/U2AF1 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP)(ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on rat liver tissue is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

Immunocytochemistry/Immunofluorescence analysis of RAMOS cells labelling U2AF35/U2AF1 with ab197591 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Flow Cytometry - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos (Human Burkitt's lymphoma) cells labeling U2AF35/U2AF1 with ab197591 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591)

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