Anti-TRA2B/SFRS10 antibody (ab31353)
Key features and details
- Rabbit polyclonal to TRA2B/SFRS10
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRA2B/SFRS10 antibody
See all TRA2B/SFRS10 primary antibodies -
Description
Rabbit polyclonal to TRA2B/SFRS10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This product was previously labelled as TRA2B
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa). Although the predicted molecular mass of TRA2B/SFRS10 is ~33 kDa, the available literature describes TRA2B/SFRS10 running at ~40 kDa in SDS-PAGE
ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. -
Tissue specificity
Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated in the RS domains.
Dimethylation at Arg-241 is probably asymmetric. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395403 Chicken
- Entrez Gene: 615156 Cow
- Entrez Gene: 6434 Human
- Entrez Gene: 20462 Mouse
- Entrez Gene: 117259 Rat
- Omim: 602719 Human
- SwissProt: Q3ZBT6 Cow
- SwissProt: P62995 Human
see all -
Alternative names
- arginine/serine-rich 10 antibody
- Arginine/serine-rich splicing factor 10 antibody
- hTRA2-beta antibody
see all
Images
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Western blot - Anti-TRA2B/SFRS10 antibody (ab31353)All lanes : Anti-TRA2B/SFRS10 antibody (ab31353) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesAlthough the predicted molecular mass of TRA2B/SFRS10 is ~33 kDa, the available literature describes TRA2B/SFRS10 running at ~40 kDa in SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-TRA2B/SFRS10 antibody (ab31353)ICC/IF image of ab31353 stained HeLa cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31353 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Western blot - Anti-TRA2B/SFRS10 antibody (ab31353)This image is courtesy of an anonymous Abreview.Anti-TRA2B/SFRS10 antibody (ab31353) at 1/100 dilution + NSC34 mouse cell lysate at 20 µg
Secondary
IRDYE® 800CW Donkey anti-Rabbit IgG at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Protocols
References (19)
ab31353 has been referenced in 19 publications.
- Park S et al. Differential Functions of Splicing Factors in Mammary Transformation and Breast Cancer Metastasis. Cell Rep 29:2672-2688.e7 (2019). PubMed: 31775037
- Cid-Samper F et al. An Integrative Study of Protein-RNA Condensates Identifies Scaffolding RNAs and Reveals Players in Fragile X-Associated Tremor/Ataxia Syndrome. Cell Rep 25:3422-3434.e7 (2018). PubMed: 30566867
- Brillen AL et al. Succession of splicing regulatory elements determines cryptic 5?ss functionality. Nucleic Acids Res 45:4202-4216 (2017). PubMed: 28039323
- Grosso F et al. Suppression of Adenovirus Replication by Cardiotonic Steroids. J Virol 91:N/A (2017). PubMed: 27881644
- Wong JJ et al. Intron retention is regulated by altered MeCP2-mediated splicing factor recruitment. Nat Commun 8:15134 (2017). WB, IP ; Mouse . PubMed: 28480880
Images
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Western blot - Anti-TRA2B/SFRS10 antibody (ab31353)All lanes : Anti-TRA2B/SFRS10 antibody (ab31353) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesAlthough the predicted molecular mass of TRA2B/SFRS10 is ~33 kDa, the available literature describes TRA2B/SFRS10 running at ~40 kDa in SDS-PAGE.
-
Immunocytochemistry/ Immunofluorescence - Anti-TRA2B/SFRS10 antibody (ab31353)
ICC/IF image of ab31353 stained HeLa cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31353 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Western blot - Anti-TRA2B/SFRS10 antibody (ab31353) This image is courtesy of an anonymous Abreview.Anti-TRA2B/SFRS10 antibody (ab31353) at 1/100 dilution + NSC34 mouse cell lysate at 20 µg
Secondary
IRDYE® 800CW Donkey anti-Rabbit IgG at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
