Anti-KHSRP antibody (ab95229)
Key features and details
- Rabbit polyclonal to KHSRP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KHSRP antibody
See all KHSRP primary antibodies -
Description
Rabbit polyclonal to KHSRP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human KHSRP aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107905) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: HeLa; HEK293; MCF7; HepG2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatChickenDogApplication Abreviews Notes WB Use a concentration of 1 mg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).Notes WB
Use a concentration of 1 mg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).Target
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Function
Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs. -
Tissue specificity
Detected in neural and non-neural cell lines. -
Sequence similarities
Belongs to the KHSRP family.
Contains 4 KH domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites. - Information by UniProt
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Database links
- Entrez Gene: 374140 Chicken
- Entrez Gene: 485022 Dog
- Entrez Gene: 8570 Human
- Entrez Gene: 16549 Mouse
- Entrez Gene: 171137 Rat
- Omim: 603445 Human
- SwissProt: Q92945 Human
- SwissProt: Q3U0V1 Mouse
see all -
Alternative names
- Far upstream element-binding protein 2 antibody
- FBP2 antibody
- FUBP2 antibody
see all
Images
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Western blot - Anti-KHSRP antibody (ab95229)All lanes : Anti-KHSRP antibody (ab95229) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using milk as the blocking agent and we expect this antibody to react with KHSRP at approximately 88 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (1)
ab95229 has been referenced in 1 publication.
- Wang F et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490
Images
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Western blot - Anti-KHSRP antibody (ab95229)All lanes : Anti-KHSRP antibody (ab95229) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using milk as the blocking agent and we expect this antibody to react with KHSRP at approximately 88 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
