Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KHSRP antibody [EPR9864] (ab150393)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9864] to KHSRP
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-KHSRP antibody [EPR9864]
    See all KHSRP primary antibodies

  • Description

    Rabbit monoclonal [EPR9864] to KHSRP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, HeLa, MCF7 and A673 cell lysates; Human brain and colon tissues; MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9864

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-KHSRP antibody [EPR9864] (ab150393)
    Western blot - Anti-KHSRP antibody [EPR9864] (ab150393)
    All lanes : Anti-KHSRP antibody [EPR9864] (ab150393) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A673 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat ani-Rabbit at 1/2000 dilution

    Predicted band size: 73 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling KHSRP with ab150393 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-KHSRP antibody [EPR9864] (ab150393)
    Flow Cytometry - Anti-KHSRP antibody [EPR9864] (ab150393)

    Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KHSRP (red) with purified ab150393 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling KHSRP with ab150393 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody [EPR9864] (ab150393)
    Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody [EPR9864] (ab150393)
    Immunofluorescent analysis of MCF7 cells labelling KHSRP with ab150393 at 1/100 dilution.
  • Anti-KHSRP antibody [EPR9864] (ab150393)
    Anti-KHSRP antibody [EPR9864] (ab150393)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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