Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-KHSRP antibody [EPR9864] (ab150393)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9864] to KHSRP
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-KHSRP antibody [EPR9864]
See all KHSRP primary antibodies
Description

Rabbit monoclonal [EPR9864] to KHSRP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293T, HeLa, MCF7 and A673 cell lysates; Human brain and colon tissues; MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9864
Isotype

IgG
Research areas

Western blot - Anti-KHSRP antibody [EPR9864] (ab150393)

All lanes : Anti-KHSRP antibody [EPR9864] (ab150393) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A673 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat ani-Rabbit at 1/2000 dilution

Predicted band size: 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling KHSRP with ab150393 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-KHSRP antibody [EPR9864] (ab150393)

Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KHSRP (red) with purified ab150393 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling KHSRP with ab150393 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody [EPR9864] (ab150393)

Immunofluorescent analysis of MCF7 cells labelling KHSRP with ab150393 at 1/100 dilution.
Anti-KHSRP antibody [EPR9864] (ab150393)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com