Anti-Munc18-1 antibody [EPR4850] (ab124920)
![Anti-Munc18-1 antibody [EPR4850] (ab124920)](https://www.abcam.com/ps/products/124/ab124920/Images/ab124920-138925-anti-munc18-1-antibody-epr4850-western-blot.jpg "Anti-Munc18-1 antibody [EPR4850] (ab124920)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4850] to Munc18-1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Munc18-1 antibody [EPR4850]
See all Munc18-1 primary antibodies -
Description
Rabbit monoclonal [EPR4850] to Munc18-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Munc18-1 aa 450-550. The exact sequence is proprietary.
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Positive control
- SH-SY5Y, HeLa, Jurkat and Y79 cell lysates; Human brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4850 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Munc18-1 antibody [EPR4850] (ab124920)All lanes : Anti-Munc18-1 antibody [EPR4850] (ab124920) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc18-1 antibody [EPR4850] (ab124920)](https://www.abcam.com/ps/products/124/ab124920/Images/ab124920-138924-anti-munc18-1-antibody-epr4850-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc18-1 antibody [EPR4850] (ab124920)ab124920, at 1/250 dilution, staining Munc18 in paraffin embedded Human brain tissue by immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Munc18-1 antibody [EPR4850] (ab124920)](https://www.abcam.com/ps/products/124/ab124920/Images/ab124920-3-benefits-of-recombinant-antibodies.png)
Anti-Munc18-1 antibody [EPR4850] (ab124920)
