Anti-Munc18-1 antibody (ab52011)
Key features and details
- Goat polyclonal to Munc18-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Munc18-1 antibody
See all Munc18-1 primary antibodies -
Description
Goat polyclonal to Munc18-1 -
Host species
Goat -
Specificity
This antibody is expected to recognize isoform a (NP_003156.1). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
C-SRVSFEDQAPTME
, corresponding to C terminal amino acids 591-603 of Human Munc18 -
Positive control
- WB: Human brain tissue lysate. IHC-P: Human cortex tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Munc18-1 antibody (ab52011)Anti-Munc18-1 antibody (ab52011) at 0.1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer)
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc18-1 antibody (ab52011)ab52011 at 5 µg/ml staining Munc18 in Human cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
