Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SND1 antibody (ab65078)

Key features and details

  • Rabbit polyclonal to SND1
  • Suitable for: IP, WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SND1 antibody
    See all SND1 primary antibodies

  • Description

    Rabbit polyclonal to SND1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Chimpanzee, Rhesus monkey, Orangutan

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human SND1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab71184.)

  • Positive control

    • This antibody gave a positive signal in the following Human Whole Cell Lysates: Ramos, HeLa - Hydroxyurea Treated (48hr, 2µM), HeLa - Staurosporine Treated (24hr, 500nM), TE 671, HeLa, JEG-3 and Jurkat.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab65078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP (1)
Use at an assay dependent concentration.
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.
Notes
IP
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Functions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2).

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Contains 4 TNase-like domains.
    Contains 1 Tudor domain.

  • Post-translational
    modifications

    Phosphorylated by PIM1 in vitro.

  • Cellular localization

    Cytoplasm. Nucleus. Melanosome. In IL-4 stimulated cells colocalizes with STAT6 in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 100 kDa coactivator antibody
    • EBNA 2 co activator (100kD) antibody
    • EBNA2 coactivator p100 antibody
    • p100 antibody
    • p100 co-activator antibody
    • p100 coactivator antibody
    • p100 EBNA2 co activator antibody
    • SND 1 antibody
    • Snd1 antibody
    • SND1_HUMAN antibody
    • Staphylococcal nuclease and tudor domain containing 1 antibody
    • Staphylococcal nuclease domain containing 1 antibody
    • Staphylococcal nuclease domain containing protein 1 antibody
    • Staphylococcal nuclease domain-containing protein 1 antibody
    • TDRD11 antibody
    • TRD 11 antibody
    • TRD11 antibody
    • Tudor domain containing protein 11 antibody
    • Tudor domain-containing protein 11 antibody
    see all

Images

  • Western blot - Anti-SND1 antibody (ab65078)
    Western blot - Anti-SND1 antibody (ab65078)
    All lanes : Anti-SND1 antibody (ab65078) at 1 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)
    Lane 3 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa

  • Immunoprecipitation - Anti-SND1 antibody (ab65078)
    Immunoprecipitation - Anti-SND1 antibody (ab65078)
    SND1 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to SND1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65078.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 102kDa: SND1.
  • Immunocytochemistry/ Immunofluorescence - Anti-SND1 antibody (ab65078)
    Immunocytochemistry/ Immunofluorescence - Anti-SND1 antibody (ab65078)
    ICC/IF image of ab65078 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65078, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, and MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 1µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab65078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab65078)
    IHC image of ab65078 staining SND1 in Human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65078, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References (7)

Publishing research using ab65078? Please let us know so that we can cite the reference in this datasheet.

ab65078 has been referenced in 7 publications.

  • Kaikkonen E  et al. The interactome of the prostate-specific protein Anoctamin 7. Cancer Biomark 28:91-100 (2020). PubMed: 32176628
  • Yu L  et al. The novel chromatin architectural regulator SND1 promotes glioma proliferation and invasion and predicts the prognosis of patients. Neuro Oncol 21:742-754 (2019). PubMed: 30753603
  • Gu X  et al. SND1 expression in breast cancer tumors is associated with poor prognosis. Ann N Y Acad Sci 1433:53-60 (2018). PubMed: 30216461
  • Shao J  et al. Aggregation of SND1 in Stress Granules is Associated with the Microtubule Cytoskeleton During Heat Shock Stimulus. Anat Rec (Hoboken) 300:2192-2199 (2017). WB, IF . PubMed: 28758359
  • Su C  et al. Phosphorylation of Tudor-SN, a novel substrate of JNK, is involved in the efficient recruitment of Tudor-SN into stress granules. Biochim Biophys Acta 1864:562-571 (2017). PubMed: 28011284
  • Carlson HL  et al. LncRNA-HIT Functions as an Epigenetic Regulator of Chondrogenesis through Its Recruitment of p100/CBP Complexes. PLoS Genet 11:e1005680 (2015). IP . PubMed: 26633036
  • Heinrich EM  et al. Regulation of miR-17-92a cluster processing by the microRNA binding protein SND1. FEBS Lett 587:2405-11 (2013). PubMed: 23770094

Images

  • Western blot - Anti-SND1 antibody (ab65078)
    Western blot - Anti-SND1 antibody (ab65078)
    All lanes : Anti-SND1 antibody (ab65078) at 1 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)
    Lane 3 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa

  • Immunoprecipitation - Anti-SND1 antibody (ab65078)
    Immunoprecipitation - Anti-SND1 antibody (ab65078)
    SND1 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to SND1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65078.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 102kDa: SND1.
  • Immunocytochemistry/ Immunofluorescence - Anti-SND1 antibody (ab65078)
    Immunocytochemistry/ Immunofluorescence - Anti-SND1 antibody (ab65078)
    ICC/IF image of ab65078 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65078, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, and MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 1µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab65078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab65078)
    IHC image of ab65078 staining SND1 in Human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65078, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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