Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-KHSRP antibody [EPR9865] (ab140648)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9865] to KHSRP
Suitable for: WB, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-KHSRP antibody [EPR9865]
See all KHSRP primary antibodies
Description

Rabbit monoclonal [EPR9865] to KHSRP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human KHSRP aa 500-600. The exact sequence is proprietary.
Positive control
- 293T, HeLa, Jurkat and A673 cell lysates. Human brain and Human colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9865
Isotype

IgG
Research areas

Western blot - Anti-KHSRP antibody [EPR9865] (ab140648)

All lanes : Anti-KHSRP antibody [EPR9865] (ab140648) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A673 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 73 kDa
Flow Cytometry - Anti-KHSRP antibody [EPR9865] (ab140648)

Overlay histogram showing Hela cells stained with ab140648 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140648, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (ab140648)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling KHSRP with ab140648 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (ab140648)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling KHSRP with ab140648 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (ab140648)

Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (ab140648)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (ab140648)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (ab140648)

Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9865] (ab140648)

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140648 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-KHSRP antibody [EPR9865] (ab140648)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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