Anti-P2Y2 antibody (ab272891)
Key features and details
- Rabbit polyclonal to P2Y2
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-P2Y2 antibody
See all P2Y2 primary antibodies -
Description
Rabbit polyclonal to P2Y2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human P2Y2. The exact sequence is proprietary. Center region.
Database link: P41231 -
Positive control
- WB: HEK-293T and A431 whole cell lysate. Mouse and rat brain tissue lysate. ICC/IF: DIV14 rat E18 primary cortical neurons cells. IP: HEK-293T whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-P2Y2 antibody (ab272891)
4% PFA-fixed DIV14 rat E18 primary cortical neurons cells stained for P2Y2 using ab272891 at 1/500 (green) dilution in ICC/IF.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500.
Blue: Fluoroshield with DAPI.
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Western blot - Anti-P2Y2 antibody (ab272891)All lanes : Anti-P2Y2 antibody (ab272891) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.10% SDS PAGE
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Immunoprecipitation - Anti-P2Y2 antibody (ab272891)Immunoprecipitation of P2Y2 protein from HEK-293T whole cell extracts using 5 μg of ab272891.
Lane 1: HEK-293T whole cell;
Lane 2: Control IgG;
Lane 3: Immunoprecipitate.
Western blot analysis was performed using ab272891, followed by anti-Rabbit IgG-HRP as a secondary reagent.
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Western blot - Anti-P2Y2 antibody (ab272891)All lanes : Anti-P2Y2 antibody (ab272891) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 50 µg per lane.10% SDS-PAGE