Anti-PTPD1/PTPN21 antibody (ab12550)
Key features and details
- Rabbit polyclonal to PTPD1/PTPN21
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PTPD1/PTPN21 antibody -
Description
Rabbit polyclonal to PTPD1/PTPN21 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
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Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.
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General notes
This product was previously labelled as PTPD1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB 1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).Notes WB
1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).Target
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Relevance
PTPD1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. The predicted PTPD1 protein has 1,174 amino acids. Its overall structure is similar to the structures of PTPH1 and PTPMEG1 (PTPN4), with an N-terminal ezrin-like domain and a single PTP domain at the extreme C terminus. Northern blot analysis shows that PTPD1 is expressed abundantly in human placenta, lung, and skeletal muscle, and to a lesser extent in several other tissues. PTPD1 may have a role in liver regeneration and spermatogenesis. -
Cellular localization
Cytoplasmic, Cytoskeleton -
Database links
- Entrez Gene: 11099 Human
- GenBank: NP_008970 Human
- Omim: 603271 Human
- SwissProt: Q16825 Human
- Unigene: 437040 Human
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Alternative names
- Protein tyrosine phosphatase D1 antibody
- Protein tyrosine phosphatase non receptor type 21 antibody
- PTPN 21 antibody
see all
Images
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Western blot - Anti-PTPD1/PTPN21 antibody (ab12550)All lanes : Anti-PTPD1/PTPN21 antibody (ab12550) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Protocols
Datasheets and documents
References (0)
ab12550 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PTPD1/PTPN21 antibody (ab12550)All lanes : Anti-PTPD1/PTPN21 antibody (ab12550) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute