Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTPD1/PTPN21 antibody (ab12550)

Key features and details

  • Rabbit polyclonal to PTPD1/PTPN21
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PTPD1/PTPN21 antibody

  • Description

    Rabbit polyclonal to PTPD1/PTPN21

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human PTPD1/PTPN21.
    (Peptide available as ab12560, ab12562)

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.

  • General notes

     This product was previously labelled as PTPD1

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab12550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
Notes
WB
1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).

Target

  • Relevance

    PTPD1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. The predicted PTPD1 protein has 1,174 amino acids. Its overall structure is similar to the structures of PTPH1 and PTPMEG1 (PTPN4), with an N-terminal ezrin-like domain and a single PTP domain at the extreme C terminus. Northern blot analysis shows that PTPD1 is expressed abundantly in human placenta, lung, and skeletal muscle, and to a lesser extent in several other tissues. PTPD1 may have a role in liver regeneration and spermatogenesis.

  • Cellular localization

    Cytoplasmic, Cytoskeleton

  • Database links

    • Alternative names

      • Protein tyrosine phosphatase D1 antibody
      • Protein tyrosine phosphatase non receptor type 21 antibody
      • PTPN 21 antibody
      • PTPN21 antibody
      • PTPNL antibody
      • PTPRL10 antibody
      • Tyrosine protein phosphatase non receptor type 21 antibody
      see all

    Images

    • Western blot - Anti-PTPD1/PTPN21 antibody (ab12550)
      Western blot - Anti-PTPD1/PTPN21 antibody (ab12550)
      All lanes : Anti-PTPD1/PTPN21 antibody (ab12550) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251)
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 133 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 40 kDa, 45 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute

    References (0)

    Publishing research using ab12550? Please let us know so that we can cite the reference in this datasheet.

    ab12550 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-PTPD1/PTPN21 antibody (ab12550)
      Western blot - Anti-PTPD1/PTPN21 antibody (ab12550)
      All lanes : Anti-PTPD1/PTPN21 antibody (ab12550) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251)
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 133 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 40 kDa, 45 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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