Anti-Connexin 32 / GJB1 antibody [2A] (ab78052)
![Anti-Connexin 32 / GJB1 antibody [2A] (ab78052)](https://www.abcam.com/ps/products/78/ab78052/Images/ab78052-393204-AntiConnexin-32-GJB1-antibody-2A-western-blot-liver-human-mouse.jpg "Anti-Connexin 32 / GJB1 antibody [2A] (ab78052)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [2A] to Connexin 32 / GJB1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Connexin 32 / GJB1 antibody [2A]
See all Connexin 32 / GJB1 primary antibodies -
Description
Mouse monoclonal [2A] to Connexin 32 / GJB1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
-
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
-
Positive control
- WB: Human, mouse and rat liver tissue lysate.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2A -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-Connexin 32 / GJB1 antibody [2A] (ab78052)All lanes : Anti-Connexin 32 / GJB1 antibody [2A] (ab78052) at 1.318 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 46,100 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThe molecular observed was in consist with published literatures (PMID 11226030, PMID: 11245683, PMID: 12403817).
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Connexin 32 / GJB1 antibody [2A] (ab78052)](https://www.abcam.com/ps/products/78/ab78052/Images/ab78052-393203-AntiConnexin-32-GJB1-antibody-2A-western-blot-liver-rat.jpg)
Western blot - Anti-Connexin 32 / GJB1 antibody [2A] (ab78052)Anti-Connexin 32 / GJB1 antibody [2A] (ab78052) at 1.318 µg/ml + Rat liver tissue lysate at 20 µg
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 46,100 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThe molecular observed was in consist with published literatures (PMID 11226030, PMID: 11245683, PMID: 12403817).
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-Connexin 32 / GJB1 antibody [2A] (ab78052)](https://www.abcam.com/ps/products/78/ab78052/Images/ab78052-3-benefits-of-recombinant-antibodies.png)
Anti-Connexin 32 / GJB1 antibody [2A] (ab78052)
