Anti-CDC42 antibody (ab155940)
Key features and details
- Rabbit polyclonal to CDC42
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Non human primates, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-CDC42 antibody
See all CDC42 primary antibodies -
Description
Rabbit polyclonal to CDC42 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Non human primates, African green monkey -
Immunogen
Truncated Human recombinant protein, corresponding to C-terminal amino acid residues in Human CDC42 (P60953).
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Positive control
- A431, C6, HepG2 and HeLa cells; Human pancreas tissue; HeLa, HepG2, 293T, A431, Jurkat, MCF7, U2-OS, K652, COS7, MEF and 3T3L1 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDC42 antibody (ab155940)All lanes : Anti-CDC42 antibody (ab155940) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : U2-OS whole cell lysate
Lane 8 : K526 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 10 : MEF whole cell lysate
Lane 11 : 3T3L1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 21 kDa
4-20% Tris HCl -
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (ab155940)Immunofluorescent analysis of A431 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown. -
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (ab155940)Immunofluorescent analysis of C6 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown. -
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (ab155940)Immunofluorescent analysis of HepG2 cells, labeling CDC42 with ab155940 at 1/200 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown. -
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (ab155940)Immunofluorescent analysis of HeLa cells, labeling CDC42 with ab155940 at 1/100 dilution. Blue: Nuclei stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody (ab155940)Immunohistochemical analysis of deparaffinised Human pancreas tissue, labeling CDC42 with ab155940 at 1/20 dilution. Detection was performed using a biotin conjugated secondary antibody and SA-HRP. Right image is immunohistochemistry performed without primary antibody (negative control).
