Human Annexin A4 ELISA Kit (ANXA4) (ab272212)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Annexin A4 ELISA Kit (ANXA4)
See all Annexin-4/ANXA4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 10 2.8% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
39.06 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 92 85% - 98% Cell Lysate 106 101% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Annexin A4 ELISA kit (ab272212) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A4 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Annexin A4 with 16 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Annexin A4 (ANXA4) is a calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Human Annexin A4 amino acids sequence is 92% identical to mouse and rat Annexin A4 sequence.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Annexin A4 (ANXA4) Capture Antibody 1 x 600µl 10X Human Annexin A4 (ANXA4) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Annexin A4 (ANXA4) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. - Information by UniProt
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Alternative names
- 35 beta calcimedin
- 35-beta calcimedin
- AIV
see all -
Database links
- Entrez Gene: 307 Human
- Omim: 106491 Human
- SwissProt: P09525 Human
- Unigene: 422986 Human
Images
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SimpleStep ELISA Protocol Diagram
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Example of human Annexin A4 standard curve in 1X Cell Extraction Buffer PTR. -
Interpolated concentrations of native Annexin A4 in human HepG2 sample based on a 1 µg/mL extract load and lung sample based on an 8 µg/mL extract load.The concentrations of Annexin A4 were measured in duplicate and interpolated from the Annexin A4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin A4 concentration was determined to be 1,767 pg/mL in HepG2 extract and 1,094 pg/mL in lung extract.
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Sandwich ELISA - Human Annexin A4 ELISA Kit (ANXA4) (ab272212)To learn more about the advantages of recombinant antibodies see here.
