Anti-Mammaglobin A antibody [SP319] - C-terminal (ab223504)
Key features and details
- Rabbit monoclonal [SP319] to Mammaglobin A - C-terminal
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mammaglobin A antibody [SP319] - C-terminal
See all Mammaglobin A primary antibodies -
Description
Rabbit monoclonal [SP319] to Mammaglobin A - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Mammaglobin A (C terminal). The exact sequence is proprietary.
Database link: Q13296 -
Positive control
- IHC-P: Human breast ductal carcinoma tissue. Flow Cyt: MCF7 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SP319 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Mammaglobin A with ab223504 at 1/100 dilution (1.08 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab223504 for 10 mins at room temperature. -
Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells stained for Mammaglobin A using ab223504 (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
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Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Mammaglobin A with ab223504 at 1/100 dilution in immunohistochemical analysis.