Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

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Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24695-36] to IL-1R-2
Suitable for: IP, Flow Cyt, WB
Reacts with: Human

Product name

Anti-IL-1R-2 antibody [EPR24695-36]
See all IL-1R-2 primary antibodies
Description

Rabbit monoclonal [EPR24695-36] to IL-1R-2
Host species

Rabbit
Tested applications

Suitable for: IP, Flow Cyt, WBmore details
Unsuitable for: ICC or IHC-P
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: SR, HDLM-2, His-tagged human IL1R2 recombinant protein (aa 14-343) lysates. Flow Cyt: HDLM-2 cells. IP: SR, HDLM-2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24695-36
Isotype

IgG

Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

All lanes : Anti-IL-1R-2 antibody [EPR24695-36] (ab273025) at 1/1000 dilution

Lane 1 : SR (human pleural effusion lymphoblast) whole cell lysate
Lane 2 : HDLM-2 (human hodgkin lymphoma ) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia monocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 45 kDa
Observed band size: 55-70 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID: 26406983).

Negative control: Jurkat and THP-1 (PMID:8702856).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: 92 seconds
Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

All lanes : Anti-IL-1R-2 antibody [EPR24695-36] (ab273025) at 1/1000 dilution

Lane 1 : His-tagged human IL1R1 recombinant protein (aa 18-332)
Lane 2 : His-tagged human IL1R2 recombinant protein (aa 14-343)

Lysates/proteins at 0.02 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 45 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This antibody specifically recognizes IL1R2 recombinant protein, but not IL1R1 recombinant protein.

Exposure time: 1 second
Flow Cytometry - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left)/ HDLM-2(Human Hodgkin lymphoma, Right) cells labelling IL-1R-2 with ab273025 at 1/50 dilution (1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat. Gated on viable cells.
Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

IL-1R-2 was immunoprecipitated from 0.35 mg SR (human pleural effusion lymphoblast) whole cell lysate 10 ug with ab273025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273025 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: SR (human pleural effusion lymphoblast) whole cell lysate 10 ug

Lane 2: ab273025 IP in SR whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273025 in SR whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds

Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.
Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

IL-1R-2 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 ug with ab273025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273025 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 ug

Lane 2: ab273025 IP in HDLM-2 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273025 in HDLM-2 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds

Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.
Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

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