Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24695-36] to IL-1R-2
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-1R-2 antibody [EPR24695-36]
    See all IL-1R-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24695-36] to IL-1R-2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SR, HDLM-2, His-tagged human IL1R2 recombinant protein (aa 14-343) lysates. Flow Cyt: HDLM-2 cells. IP: SR, HDLM-2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24695-36

  • Isotype

    IgG

Images

  • Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)
    Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)
    All lanes : Anti-IL-1R-2 antibody [EPR24695-36] (ab273025) at 1/1000 dilution

    Lane 1 : SR (human pleural effusion lymphoblast) whole cell lysate
    Lane 2 : HDLM-2 (human hodgkin lymphoma ) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 45 kDa
    Observed band size: 55-70 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26406983).

    Negative control: Jurkat and THP-1 (PMID:8702856).

    Samples are non-boiled as boiling may cause protein aggregates.

     Exposure time: 92 seconds

     

  • Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)
    Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)
    All lanes : Anti-IL-1R-2 antibody [EPR24695-36] (ab273025) at 1/1000 dilution

    Lane 1 : His-tagged human IL1R1 recombinant protein (aa 18-332)
    Lane 2 : His-tagged human IL1R2 recombinant protein (aa 14-343)

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 45 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody specifically recognizes IL1R2 recombinant protein, but not IL1R1 recombinant protein.

    Exposure time: 1 second

     

  • Flow Cytometry - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)
    Flow Cytometry - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left)/ HDLM-2(Human Hodgkin lymphoma, Right) cells labelling IL-1R-2 with ab273025 at 1/50 dilution (1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat. Gated on viable cells.

  • Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)
    Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    IL-1R-2 was immunoprecipitated from 0.35 mg SR (human pleural effusion lymphoblast) whole cell lysate 10 ug with ab273025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273025 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: SR (human pleural effusion lymphoblast) whole cell lysate 10 ug

    Lane 2: ab273025 IP in SR whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273025 in SR whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.

  • Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)
    Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    IL-1R-2 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 ug with ab273025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273025 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 ug

    Lane 2: ab273025 IP in HDLM-2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273025 in HDLM-2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.

  • Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)
    Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

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