Anti-GRP94 antibody (ab230842)
Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-GRP94 antibody
See all GRP94 primary antibodies -
Description
Rabbit polyclonal to GRP94 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Chinese hamster
Predicted to work with: Rat, Chicken, Cow, Pig, Monkey
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Immunogen
Synthetic peptide within GRP94 aa 16-43 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P14625 -
Positive control
- IHC-P: Human testis and liver tissues. ICC/IF: NCI-H460 cells. WB: HepG2, CHO-K1, K562 and HeLa whole cell lysate (ab150035); Mouse liver lysate. Flow Cyt: K562 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRP94 antibody (ab230842)All lanes : Anti-GRP94 antibody (ab230842) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : CHO-K1 (chinese hamster ovary cell line) cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 92 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab230842)Formalin-fixed, paraffin-embedded human testis tissue stained for GRP94 using ab230842 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab230842)Formalin-fixed, paraffin-embedded human liver tissue stained for GRP94 using ab230842 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab230842)NCI-H460 (human lung cancer cell line) cells stained for GRP94 (green) using ab230842 at 1/10 dilution in confocal ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used to stain the cell nuclear (blue).
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Flow Cytometry - Anti-GRP94 antibody (ab230842)Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling GRP94 with ab230842 at 1/10 dilution (green) compared with a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
