Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRP94 antibody [EPR3988] (ab108606)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3988] to GRP94
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-GRP94 antibody [EPR3988]
    See all GRP94 primary antibodies

  • Description

    Rabbit monoclonal [EPR3988] to GRP94

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, T47D, HeLa, K-562, Jurkat and HepG2 cell lysates. IHC-P: Human breast and gastric adenocarcinoma tissue.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GRP94 antibody [EPR3988] (ab108606)
    Western blot - Anti-GRP94 antibody [EPR3988] (ab108606)
    All lanes : Anti-GRP94 antibody [EPR3988] (ab108606) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : HSP90B1 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 92 kDa
    Observed band size: 94 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab108606 observed at 94 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab108606 Anti-GRP94 antibody [EPR3988] was shown to specifically react with GRP94 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266313 (knockout cell lysate ab257254) was used. Wild-type and GRP94 knockout samples were subjected to SDS-PAGE. ab108606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)

    Ab108606 staining GRP94 in paraffin embedded Human hepatocellular carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:1000 dilution (0.57µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on human hepatocellular carcinoma.

  • Western blot - Anti-GRP94 antibody [EPR3988] (ab108606)
    Western blot - Anti-GRP94 antibody [EPR3988] (ab108606)
    All lanes : Anti-GRP94 antibody [EPR3988] (ab108606) at 1/1000 dilution

    Lane 1 : T47D cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 92 kDa
    Observed band size: 94 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)

    Ab108606 staining GRP94 in paraffin embedded Human lung carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:1000 dilution (0.57µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on human lung carcinoma. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)

    Ab108606 staining GRP94 in paraffin embedded Mouse liver carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:1000 dilution (0.57µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse liver.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)
    ab108606, at 1/100 dilution, staining GRP94 in paraffin-embedded Human breast tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] (ab108606)
    ab108606, at 1/100 dilution, staining GRP94 in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
  • Anti-GRP94 antibody [EPR3988] (ab108606)
    Anti-GRP94 antibody [EPR3988] (ab108606)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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