Anti-GRP94 antibody (ab227293)
Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRP94 antibody
See all GRP94 primary antibodies -
Description
Rabbit polyclonal to GRP94 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
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Immunogen
Recombinant fragment within Human GRP94 (internal sequence). The exact sequence is proprietary.
Database link: P14625 -
Positive control
- WB: NIH/3T3, NCI-H1299 and HeLa S3 whole cell lysates. IP: HeLa whole cell extract. IHC-P: SCM-1 xenograft tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRP94 antibody (ab227293)All lanes : Anti-GRP94 antibody (ab227293) at 1/2000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : GRP94 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 92 kDa
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Western blot - Anti-GRP94 antibody (ab227293)Anti-GRP94 antibody (ab227293) at 1/5000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 92 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-GRP94 antibody (ab227293)All lanes : Anti-GRP94 antibody (ab227293) at 1/1000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 92 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-GRP94 antibody (ab227293)GRP94 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 5 μg ab227293. Western blot was performed from the immunoprecipitate using ab227293.
Lane 1: HeLa whole cell extract (Input).
Lane 2: Control IgG instead of ab227293 in HeLa whole cell extract.
Lane 3: ab227293 IP in HeLa whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab227293)Paraffin-embedded SCM-1 xenograft tissue stained for GRP94 using ab227293 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab227293)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRP94 (green) using ab227293 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (bue).
