Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3988] to GRP94 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-GRP94 antibody [EPR3988] - BSA and Azide free
    See all GRP94 primary antibodies

  • Description

    Rabbit monoclonal [EPR3988] to GRP94 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T47D, HeLa, HEK293T, K-562, Jurkat and HepG2 cell lysates. IHC-P: Human breast and gastric adenocarcinoma tissue.

  • General notes

    ab238959 is the carrier-free version of ab108606 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238959 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)
    Western blot - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)
    All lanes : Anti-GRP94 antibody [EPR3988] (ab108606) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : HSP90B1 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 92 kDa
    Observed band size: 94 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab108606).

    Lanes 1-3: Merged signal (red and green). Green - ab108606 observed at 94 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab108606 Anti-GRP94 antibody [EPR3988] was shown to specifically react with GRP94 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266313 (knockout cell lysate ab257254) was used. Wild-type and GRP94 knockout samples were subjected to SDS-PAGE. ab108606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

    Ab108606 staining GRP94 in paraffin embedded Mouse liver carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:1000 dilution (0.57µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse liver.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108606).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

    Ab108606 staining GRP94 in paraffin embedded Human lung carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:1000 dilution (0.57µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on human lung carcinoma. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108606).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

    ab108606, at 1/100 dilution, staining GRP94 in paraffin-embedded Human breast tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108606).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

    ab108606, at 1/100 dilution, staining GRP94 in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108606).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

    ab108606 staining GRP94 in paraffin embedded human hepatocellular carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections).

    Samples are incubated in primary antibody at 1:1000 dilution (0.57 µg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Cytoplasmic staining on human hepatocellular carcinoma.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108606).

  • Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)
    Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

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