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Anti-GRP94 antibody (ab223371)

Anti-GRP94 antibody (ab223371)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GRP94
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GRP94 antibody
    See all GRP94 primary antibodies
  • Description

    Rabbit polyclonal to GRP94
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse GRP94 (C terminal). Corresponding to the sequence near the C-terminus.
    Database link: P08113

  • Positive control

    • WB: Rat brain cell lysate. ICC/IF: Heat shocked HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab223371)
    Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab223371)

    2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRP94 (red) using ab223371 at 1/120 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit APC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.

    (A) DAPI nuclear stain (B) ab223371 (C) Merge.

  • Western blot - Anti-GRP94 antibody (ab223371)
    Western blot - Anti-GRP94 antibody (ab223371)
    Anti-GRP94 antibody (ab223371) at 1/250 dilution + Rat brain cell lysate at 20 µg

    Predicted band size: 93 kDa
    Observed band size: 50,75,94-100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    Blocking buffer: 5% milk in TBST.

    Color Development: TMB solution.

    Primary antibody was incubated for 1 hour at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab223371)
    Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab223371)

    2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GRP94 (green) using ab223371 at 1/120 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit FITC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.

    (A) DAPI nuclear stain (B) ab223371 (C) Merge.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-GRP94 antibody [EPR3988] (ab108606)

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    Anti-GRP94 antibody (ab3674)

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    Alexa Fluor® 647 Anti-GRP94 antibody [EPR3988] (ab199206)

    Applications: ICC/IF

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    Anti-GRP94 antibody [EPR3988] - BSA and Azide free (ab238959)

    Applications: IHC-P, WB

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    Applications: ICC/IF

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