Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)
![Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-451394-anti-grp94-antibody-cl2647-c-terminal-western-blot-hela-hek293-a431-hepg2-human.jpg "Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)")
Key features and details
- Mouse monoclonal [CL2647] to GRP94 - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-GRP94 antibody [CL2647] - C-terminal
See all GRP94 primary antibodies -
Description
Mouse monoclonal [CL2647] to GRP94 - C-terminal -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment (proprietary-tag) corresponding to Human GRP94 aa 650-800 (C terminal).
Database link: P14625 -
Epitope
Binds to an epitope located within the peptide sequence EEEPEETAED as determined by overlapping synthetic peptides. -
Positive control
- WB: HeLa, A431, HEK293, and HepG2 lysates; ICC: U251, HeLa, A431, MCF7 and U2OS cells; IHC-P: Human liver, Human prostate, Human cortex and Human rectum tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL2647 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)All lanes : Anti-GRP94 antibody [CL2647] - C-terminal (ab210960) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Predicted band size: 92 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258450-anti-grp94-antibody-cl2647-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunofluorescence analysis of U251 cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258440-anti-grp94-antibody-cl2647-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate tissue labeling GRP94 with ab210960 at 1/5000 dilution.
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![Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-281861-anti-grp94-antibody-cl2647-c-terminal-western-blot.jpg)
Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)All lanes : Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)
Lane 1 : U-251 cells, transfected with control siRNA
Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
Lane 3 : U-251 cells, transfected with target specific siRNA probe #2
Predicted band size: 92 kDaDownregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
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![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258449-anti-grp94-antibody-cl2647-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunofluorescence analysis of U2OS cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258448-anti-grp94-antibody-cl2647-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunofluorescence analysis of MCF7 cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258447-anti-grp94-antibody-cl2647-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunofluorescence analysis of A431 cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258446-anti-grp94-antibody-cl2647-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunofluorescence analysis of HeLa cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258443-anti-grp94-antibody-cl2647-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human liver tissue labeling GRP94 with ab210960 at 1/5000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258442-anti-grp94-antibody-cl2647-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human rectum tissue labeling GRP94 with ab210960 at 1/5000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)](https://www.abcam.com/ps/products/210/ab210960/Images/ab210960-258441-anti-grp94-antibody-cl2647-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human cortex tissue labeling GRP94 with ab210960 at 1/5000 dilution.
