Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4236] to GST - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Species independent

Overview

  • Product name

    Anti-GST antibody [EPR4236] - BSA and Azide free
    See all GST primary antibodies

  • Description

    Rabbit monoclonal [EPR4236] to GST - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide (GST-tag) within GST aa 300-450. The exact sequence is proprietary. GST tag was fused to ATG16L1 to produce a recombinant protein.
    Database link: Q676U5

  • General notes

    Ab235384 is the carrier-free version of ab111947. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab235384 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
    Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

    (Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

    Lane 1: Primary: ab180123, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
    Lane 2: Primary: ab180123, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate
    Secondary: ab131366, Veriblot, 1/10000 dilution
    Lane 3: Primary: ab172730, Rabbit monoclonal IgG
    Sample: 293T transfected with GST tagged protein vector cell lysate

    All lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).

  • Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
    Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

    GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

    Lane 1: Primary: ab111947, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
    Lane 2: Primary: ab111947, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate
    Lane 3: Primary: ab172730, Rabbit monoclonal IgG
    Sample: 293T transfected with GST tagged protein vector cell lysate

    All lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).

  • Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
    Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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