Mitochondrial Superoxide Indicator Assay Kit (Red) (ab228567)
Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Fluorescence microscope
- Sample type: Adherent cells
Overview
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Product name
Mitochondrial Superoxide Indicator Assay Kit (Red) -
Detection method
Fluorescent -
Sample type
Adherent cells -
Assay type
Cell-based -
Product overview
ab228567 Mitochondrial Superoxide Indicator (Red) Assay Kit utilizes a sensitive detection reagent for mitochondrial superoxide that rapidly partitions to the mitochondria where it is oxidized by superoxide and exhibits red fluorescence. The reagent is resistant to oxidation by other ROS systems and is selective only for superoxide.
This kit may be useful for the investigation of agents that modulate oxidative stress found in various disease states.
The protocol provides enough reagents to analyze 10 x 96 wells (960 samples) using the methods described.
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Notes
Superoxide occurs in mitochondria as a result of incompletely reduced oxygen being produced during oxidative phosphorylation and going on to interact with molecular oxygen and forming superoxide. Increases in cellular superoxide production have been linked to cardiovascular diseases such as hypertension and atherosclerosis and in neurodegenerative disorders such as Parkinson's, Alzheimer's, and amyotrophic lateral sclerosis (ALS).
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Platform
Fluorescence microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 1 kit 5X Live Cell Imaging Buffer with Hoechst 33342 1 x 20ml DMSO 1 x 500µl HBSS Buffer 1 x 100ml Superoxide Detection Agent 2 x 5 units
Images
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HeLa cells labeled with ab228567 Mitochondrial Superoxide Indicator Assay Kit
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with Mitochondrial superoxide indicator (red).