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Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)

Key features and details

  • Alkaline Phosphatase Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: WB
  • Reacts with: Species independent
  • Conjugation: Alkaline Phosphatase
  • Isotype: IgG1

Overview

  • Product name

    Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies

  • Description

    Alkaline Phosphatase Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)

  • Host species

    Mouse

  • Conjugation

    Alkaline Phosphatase

  • Specificity

    ab81655 recognizes the DYKDDDDK tag sequence (also known as the FLAG® tag sequence) at an N-terminal, Met-N-terminal, C-terminal, or internal site.

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.013% Sodium azide
    Constituents: 0.3025% Tris, 0.87% Sodium chloride
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    M2

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
    Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
    All lanes : Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655) at 1/10000 dilution

    Lane 1 : 60 kDa DYKDDDDK-tagged protein at 0.3 µg
    Lane 2 : 60 kDa DYKDDDDK-tagged protein at 0.01 µg
    Lane 3 : 60 kDa DYKDDDDK-tagged protein at 0.03 µg
    Lane 4 : 60 kDa DYKDDDDK-tagged protein at 0.001 µg
    Lane 5 : 60 kDa DYKDDDDK-tagged protein at 0.003 µg


    Imaged using stabilized Alkaline Phosphatase substrate.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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