Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)

Key features and details

  • Mouse monoclonal [F-tag-01] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: ICC, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG1

Overview

  • Product name

    Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies

  • Description

    Mouse monoclonal [F-tag-01] to DDDDK tag (Binds to FLAG® tag sequence)

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK293 cells transfected with vector overexpressing DDDDK tag; ICC/ IF: transfected HEK293 cells.

  • General notes

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    F-tag-01

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
    Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230) Image courtesy of an anonymous AbReview

    ab18230 staining DDDDK tag in Human HeLa, cervical carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% tween20 and blocked with 0.5% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 24 hours at 4°C. Ab8517 (1/200) was used as the secondary antibody

    See Abreview

  • Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
    Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
    (a) Fusion of nuclear protein stained with ab18230 (red)
    (b) Cell nuclei stained with DAPI (blue)
    (c) Merge of (a) and (b) confirming nuclear localisation of the fusion protein.
  • Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
    Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230) Image from Ögmundsdóttir MH et al., PLoS One. 2012;7(5):e36616. Epub 2012 May 4. Fig 1.; doi:10.1371/journal.pone.0036616; May 4, 2012, PLoS ONE 7(5): e36616.
    Immunofluorescence analysis of transfected HEK293 cells, staining DDDDK tag with ab18230 at 1/200 dilution.

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