Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
![Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)](https://www.abcam.com/ps/products/18/ab18230/Images/ab18230-263421-anti-ddddk-tag-antibody-f-tag-01-equivalent-to-flag-antibodies-from-sigma-immunofluorescence.jpg "Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)")
Key features and details
- Mouse monoclonal [F-tag-01] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ICC, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
Mouse monoclonal [F-tag-01] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Mouse -
Tested applications
Suitable for: ICC, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
Sequence:DYKDDDDK
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Positive control
- WB: HEK293 cells transfected with vector overexpressing DDDDK tag; ICC/ IF: transfected HEK293 cells.
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General notes
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
F-tag-01 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230) Image courtesy of an anonymous AbReview
ab18230 staining DDDDK tag in Human HeLa, cervical carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% tween20 and blocked with 0.5% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 24 hours at 4°C. Ab8517 (1/200) was used as the secondary antibody
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![Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)](https://www.abcam.com/ps/products/18/ab18230/Images/ab18230-27530-anti-ddddk-tag-antibody-f-tag-01-equivalent-to-flag-antibodies-from-sigma-immunocytochemistry.jpg)
Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)(a) Fusion of nuclear protein stained with ab18230 (red)
(b) Cell nuclei stained with DAPI (blue)
(c) Merge of (a) and (b) confirming nuclear localisation of the fusion protein. -
![Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)](https://www.abcam.com/ps/products/18/ab18230/Images/ab18230-27528-anti-ddddk-tag-antibody-f-tag-01-equivalent-to-flag-antibodies-from-sigma-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230) Image from Ögmundsdóttir MH et al., PLoS One. 2012;7(5):e36616. Epub 2012 May 4. Fig 1.; doi:10.1371/journal.pone.0036616; May 4, 2012, PLoS ONE 7(5): e36616.Immunofluorescence analysis of transfected HEK293 cells, staining DDDDK tag with ab18230 at 1/200 dilution.
