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Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: WB, ELISA, Dot blot, IP, In situ hybridization
  • Reacts with: Species independent
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
  • Description

    Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
  • Host species

    Mouse
  • Specificity

    Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged (also known as the FLAG® tag sequence) fusion proteins.

  • Tested applications

    Suitable for: WB, ELISA, Dot blot, IP, In situ hybridizationmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HEK293 cells transfected with DYKDDDDK (also known as the FLAG® tag sequence).
  • General notes

    If stored at 4°C please add 0.1% sodium azide.

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab125243 was purified by protein A affinity chromatography from TCS
  • Clonality

    Monoclonal
  • Clone number

    FG4R
  • Isotype

    IgG2b
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • DDDDK Tag

Images

  • Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)
    Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)
    All lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate non-transfected
    Lane 2 : HEK293 cell lysate transfected with DDDDK tagged protein vector

    Developed using the ECL technique.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)

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    Applications: ELISA, ICC, IHC-P, WB

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