Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)
Key features and details
- Rat monoclonal [6F7] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
Rat monoclonal [6F7] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Rat -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence). DDDDK peptide
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General notes
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
ab213519 is purified from culture supernatant of hybridoma cells grown in a bioreactor. -
Clonality
Monoclonal -
Clone number
6F7 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519) at 2 µg/ml
Lane 1 : N-terminal-DDDDK-tagged BAP protein at 0.05 µg
Lane 2 : N-terminal-DDDDK-tagged BAP protein at 0.025 µg
Lane 3 : N-terminal-DDDDK-tagged BAP protein at 0.01 µg
Lane 4 : N-terminal-DDDDK-tagged BAP protein at 0.005 µg
Lane 5 : C-terminal-DDDDK-tagged BAP protein at 0.05 µg
Lane 6 : C-terminal-DDDDK-tagged BAP protein at 0.025 µg
Lane 7 : C-terminal-DDDDK-tagged BAP protein at 0.01 µg
Lane 8 : C-terminal-DDDDK-tagged BAP protein at 0.005 µg
Secondary
All lanes : Rabbit Anti-Rat IgG-Peroxidase
Developed using the ECL technique.
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Lane 1 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519) at 1 µg/ml
Lane 2 : Negative control- No Primary antibody
All lanes : HEK-293T cell lysate overexpressing Human Rab25-DDDDK
Secondary
All lanes : Rabbit Anti-Rat IgG-Peroxidase
Developed using the ECL technique.
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ab213519 was used to immunoprecipitate BACE1 from a whole extract of HEK-293T cells overexpressing human BACE1-DDDDK-tagged protein. Detection antibody: Rabbit Anti-DDDDK followed by Monoclonal Anti-Rabbit IgG native-peroxidase and a chemiluminescent substrate.
Lanes
1. 10 μg antibody
2. 2.5 μg antibody
3. Negative control: 10 μg nonrelevant antibody
Lane 1 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519) at 10 µg/ml
Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519) at 2.5 µg/ml
Lane 3 : Negative control - nonrelevant antibody at 10 µg/ml
All lanes : HEK-293T cells overexpressing Human BACE1-DDDDK-tagged protein
Secondary
All lanes : Anti-Rabbit IgG native-peroxidase
Developed using the ECL technique.