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Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)

Price and availability

328 339 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rat monoclonal [6F7] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: WB, IP
  • Reacts with: Species independent
  • Isotype: IgG1

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Overview

  • Product name

    Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
  • Description

    Rat monoclonal [6F7] to DDDDK tag (Binds to FLAG® tag sequence)
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence). DDDDK peptide

  • General notes

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Purification notes

    ab213519 is purified from culture supernatant of hybridoma cells grown in a bioreactor.
  • Clonality

    Monoclonal
  • Clone number

    6F7
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • DDDDK Tag

Images

  • Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)
    Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)
    All lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519) at 2 µg/ml

    Lane 1 : N-terminal-DDDDK-tagged BAP protein at 0.05 µg
    Lane 2 : N-terminal-DDDDK-tagged BAP protein at 0.025 µg
    Lane 3 : N-terminal-DDDDK-tagged BAP protein at 0.01 µg
    Lane 4 : N-terminal-DDDDK-tagged BAP protein at 0.005 µg
    Lane 5 : C-terminal-DDDDK-tagged BAP protein at 0.05 µg
    Lane 6 : C-terminal-DDDDK-tagged BAP protein at 0.025 µg
    Lane 7 : C-terminal-DDDDK-tagged BAP protein at 0.01 µg
    Lane 8 : C-terminal-DDDDK-tagged BAP protein at 0.005 µg

    Secondary
    All lanes : Rabbit Anti-Rat IgG-Peroxidase

    Developed using the ECL technique.
  • Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)
    Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)
    Lane 1 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519) at 1 µg/ml
    Lane 2 : Negative control- No Primary antibody

    All lanes : HEK-293T cell lysate overexpressing Human Rab25-DDDDK

    Secondary
    All lanes : Rabbit Anti-Rat IgG-Peroxidase

    Developed using the ECL technique.
  • Immunoprecipitation - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)
    Immunoprecipitation - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519)

    ab213519 was used to immunoprecipitate BACE1 from a whole extract of HEK-293T cells overexpressing human BACE1-DDDDK-tagged protein. Detection antibody: Rabbit Anti-DDDDK followed by Monoclonal Anti-Rabbit IgG native-peroxidase and a chemiluminescent substrate.
    Lanes
    1. 10 μg antibody
    2. 2.5 μg antibody
    3. Negative control: 10 μg nonrelevant antibody



    Lane 1 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519) at 10 µg/ml
    Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [6F7] (ab213519) at 2.5 µg/ml
    Lane 3 : Negative control - nonrelevant antibody at 10 µg/ml

    All lanes : HEK-293T cells overexpressing Human BACE1-DDDDK-tagged protein

    Secondary
    All lanes : Anti-Rabbit IgG native-peroxidase

    Developed using the ECL technique.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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