HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
![HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)](https://www.abcam.com/ps/products/49/ab49763/Images/ab49763-260172-anti-ddddk-tag-antibody-m2-equivalent-to-flag-antibodies-from-sigma-hrp-western-blot.jpg "HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)")
Key features and details
- HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
Sequence:DYKDDDDK
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General notes
This product was changed from ascites to tissue culture supernatant on 3rd December 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Properties
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Form
Lyophilized:Reconstitute to 1.0 ml with distilled deionized water vortex gently and allow vial to sit on ice for 20 minutes. Store at +4°C. Do not freeze. -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.64% Sodium phosphate, 0.58% Sodium chloride, 1.5% BSA, 1.71% Sucrose -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) This image is courtesy of an anonymous AbreviewAll lanes : HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) at 1/3000 dilution
All lanes : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 45 secondsSample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.
5% milk blocking was used for 1 hour.
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![ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)](https://www.abcam.com/ps/products/49/ab49763/Images/ab49763-260171-anti-ddddk-tag-antibody-m2-equivalent-to-flag-antibodies-from-sigma-hrp-elisa.jpg)
ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) This image is courtesy of an anonymous AbreviewELISA for the detection of FLAG®-tag purified protein from Escherichia coli, using ab28947 (1/5000). Please refer to abreview for further experimental details.
Please note that in order to show a clearer image, we have brightened the original image.
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![Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)](https://www.abcam.com/ps/products/49/ab49763/Images/ab49763-61825-anti-ddddk-tag-antibody-m2-equivalent-to-flag-antibodies-from-sigma-hrp-western-blot.jpg)
Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) This image is courtesy of an anonymous AbreviewSaccharomyces cerevisiae purified FLAG®-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.
