Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)

Key features and details

  • HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies

  • Description

    HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    This product was changed from ascites to tissue culture supernatant on 3rd December 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Properties

  • Form

    Lyophilized:Reconstitute to 1.0 ml with distilled deionized water vortex gently and allow vial to sit on ice for 20 minutes. Store at +4°C. Do not freeze.

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.64% Sodium phosphate, 0.58% Sodium chloride, 1.5% BSA, 1.71% Sucrose
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    M2

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) This image is courtesy of an anonymous Abreview
    All lanes : HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) at 1/3000 dilution

    All lanes : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 45 seconds


    Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.

    5% milk blocking was used for 1 hour.

    See Abreview

  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) This image is courtesy of an anonymous Abreview

    ELISA for the detection of FLAG®-tag purified protein from Escherichia coli, using ab28947 (1/5000). Please refer to abreview for further experimental details.

    Please note that in order to show a clearer image, we have brightened the original image.

    See Abreview

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) This image is courtesy of an anonymous Abreview

    Saccharomyces cerevisiae purified FLAG®-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)

© 2021 Astana Biomed Group LLP. All rights reserved.