Anti-eIF-6 antibody [EPR6512(B)] (ab124839)
![Anti-eIF-6 antibody [EPR6512(B)] (ab124839)](https://www.abcam.com/ps/products/124/ab124839/Images/ab124839-138797-anti-eif-6-antibody-epr6512b-western-blot.jpg "Anti-eIF-6 antibody [EPR6512(B)] (ab124839)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6512(B)] to eIF-6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-eIF-6 antibody [EPR6512(B)]
See all eIF-6 primary antibodies -
Description
Rabbit monoclonal [EPR6512(B)] to eIF-6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
corresponding to Human eIF-6 aa 150-250.
Database link: P56537 -
Positive control
- HeLa, RAW 264.7, K562, 293T, and HepG2 cell lysates and Human colonic adenocarcinoma tissue.
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General notes
Previously labelled as Integrin beta 4 binding protein.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Not purified; provided in cell supernatant and storage buffer. -
Clonality
Monoclonal -
Clone number
EPR6512(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF-6 antibody [EPR6512(B)] (ab124839)All lanes : Anti-eIF-6 antibody [EPR6512(B)] (ab124839) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF-6 antibody [EPR6512(B)] (ab124839)](https://www.abcam.com/ps/products/124/ab124839/Images/ab124839-138796-anti-eif-6-antibody-epr6512b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF-6 antibody [EPR6512(B)] (ab124839)ab124839, at 1/100 to 1/250, staining eIF-6 in Human colonic adenocarcinoma by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-eIF-6 antibody [EPR6512(B)] (ab124839)](https://www.abcam.com/ps/products/124/ab124839/Images/ab124839-3-benefits-of-recombinant-antibodies.png)
Anti-eIF-6 antibody [EPR6512(B)] (ab124839)
