Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF-6 antibody (ab77298)

Key features and details

  • Rabbit polyclonal to eIF-6
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-eIF-6 antibody
    See all eIF-6 primary antibodies

  • Description

    Rabbit polyclonal to eIF-6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow

  • Immunogen

    Synthetic peptide corresponding to Human eIF-6 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90213)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: SW480; Caco2; HeLa; HEK293; HepG2; LOVO; K562.

  • General notes

    Previously labelled as Integrin beta 4 binding protein. 

Properties

Applications

Our Abpromise guarantee covers the use of ab77298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.

  • Tissue specificity

    Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).

  • Sequence similarities

    Belongs to the eIF-6 family.

  • Post-translational
    modifications

    Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.

  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • b(2)gcn antibody
    • B(2)GCN homolog antibody
    • B4 integrin interactor antibody
    • Binding protein of beta-4 integrin antibody
    • CAB antibody
    • eIF-6 antibody
    • EIF3A antibody
    • EIF6 antibody
    • Eukaryotic translation initiation factor 3A antibody
    • Eukaryotic translation initiation factor 6 antibody
    • IF6_HUMAN antibody
    • Integrin beta 4 binding protein antibody
    • ITGB4BP antibody
    • OK/SW-cl.27 antibody
    • p27 beta 4 integrin binding protein antibody
    • p27(BBP) antibody
    • p27BBP antibody
    • RP4-614O4.1 antibody
    see all

Images

  • Western blot - Anti-eIF-6 antibody (ab77298)
    Western blot - Anti-eIF-6 antibody (ab77298)
    All lanes : Anti-eIF-6 antibody (ab77298) at 1 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF-6 antibody (ab77298)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF-6 antibody (ab77298)
    ICC/IF image of ab77298 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77298, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, and MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 1µg/ml.

References (1)

Publishing research using ab77298? Please let us know so that we can cite the reference in this datasheet.

ab77298 has been referenced in 1 publication.

  • Tarallo R  et al. The nuclear receptor ERß engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading. Genome Biol 18:189 (2017). WB ; Human . PubMed: 29017520

Images

  • Western blot - Anti-eIF-6 antibody (ab77298)
    Western blot - Anti-eIF-6 antibody (ab77298)
    All lanes : Anti-eIF-6 antibody (ab77298) at 1 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF-6 antibody (ab77298)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF-6 antibody (ab77298)
    ICC/IF image of ab77298 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77298, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, and MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 1µg/ml.

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